International Journal of Applied and Basic Medical Research

ORIGINAL ARTICLE
Year
: 2020  |  Volume : 10  |  Issue : 4  |  Page : 234--239

Molecular detection of fusion oncogenes in zambian patients with acute lymphoblastic leukemia


Pauline Okuku1, Geoffrey Kwenda2, Mulemba Samutela2, Panji Nkhoma2, Hamakwa Mantina4 
1 Department of Pathology and Microbiology, School of Medicine, University of Zambia; Department of Pathology and Microbiology, University Teaching Hospital, Lusaka, Zambia
2 Department of Biomedical Sciences, School of Health Sciences, University of Zambia, Lusaka, Zambia

Correspondence Address:
Pauline Okuku
Department of Pathology and Microbiology, School of Medicine, University of Zambia, P. O. Box: 50110, Lusaka 15101
Zambia

Introduction: Chromosomal aberrations play a significant role in the pathogenesis of acute lymphoblastic leukemia (ALL) with prognostic and therapeutic implications. Despite the availability of molecular tools, low-resource settings struggle to diagnose the disease due to limited diagnostic capacity. The objective of this study was to detect common chromosomal aberrations in patients with ALL attending the University Teaching Hospital (UTH) in Lusaka, Zambia. Materials and Methods: In this prospective study, 19 blood samples from patients with ALL were screened for the presence of BCR-ABL, E2A-PBX1, MLL-AF4, and ETV6-RUNX1 fusion oncogenes using reverse transcriptase-polymerase chain reaction assay. Blood counts and clinical characteristics of patients were also assessed. Results: The age of patients ranged from 1½ to 72 years and comprised 57.9% of males and 42.1% of females. The majority of these patients were children (68%), and adults only comprised 32%. Only BCR-ABL and E2A-PBX1 oncogenes were detected in 3/19 of cases. The BCR-ABL gene was detected in a 4-year-old female child and a 15-year-old child. Both cases were associated with hepatomegaly and anemia coupled with low hemoglobin, white blood cell, and platelet counts. E2A-PBX1 was detected in a 12-year-old child with lymphadenopathy and splenomegaly, coupled with low hemoglobin, white blood cell, and platelet counts. All the three patients who harbored these fusion oncogenes died. Conclusion: This is the first study from Zambia to investigate the presence of fusion oncogenes in leukemia patients, which were found only among the older children population. Based on these findings, we recommend that molecular diagnosis be made a priority for the younger leukemia patient population at UTH.


How to cite this article:
Okuku P, Kwenda G, Samutela M, Nkhoma P, Mantina H. Molecular detection of fusion oncogenes in zambian patients with acute lymphoblastic leukemia.Int J App Basic Med Res 2020;10:234-239


How to cite this URL:
Okuku P, Kwenda G, Samutela M, Nkhoma P, Mantina H. Molecular detection of fusion oncogenes in zambian patients with acute lymphoblastic leukemia. Int J App Basic Med Res [serial online] 2020 [cited 2020 Nov 28 ];10:234-239
Available from: https://www.ijabmr.org/article.asp?issn=2229-516X;year=2020;volume=10;issue=4;spage=234;epage=239;aulast=Okuku;type=0