International Journal of Applied and Basic Medical Research

ORIGINAL ARTICLE
Year
: 2017  |  Volume : 7  |  Issue : 4  |  Page : 243--246

p53 codon 72 gene polymorphism studies and p53 expression by immunohistochemistry in oral lesions as risk factor for malignancy


Nishi Tandon1, Anand Narain Srivastava1, Naseem Fatima1, Syed Tasleem Raza2, Vijay Kumar3 
1 Department of Pathology, Era's Lucknow Medical College and Hospital, Lucknow, Uttar Pradesh, India
2 Department of Biochemistry, Era's Lucknow Medical College and Hospital, Lucknow, Uttar Pradesh, India
3 Department of Surgical Oncology, K. G. Medical University, Lucknow, Uttar Pradesh, India

Correspondence Address:
Dr. Nishi Tandon
Department of Pathology, Era's Lucknow Medical College and Hospital, Lucknow - 226 003, Uttar Pradesh
India

Background: Wild-type p53 nuclear phosphoproteins are critical cell cycle regulatory tumor-suppressor gene. Genetic mutation of p53 gene is common in several head–neck cancers, usually associated with smoking and human papillomavirus (HPV) infection. In India, instead of HPV, tobacco/pan masala chewing is more commonly associated with oral cancer. Aim: The aim of this study was to investigate p53 codon 72 gene polymorphism and expression of p53 by immunohistochemistry (IHC) in oral lesions as a risk factor for its association with malignancy. Materials and Methods: A total of 41 cases of oral lesions comprising 6 cases of leukoplakia and 35 cases of oral squamous cell carcinoma (OSCC), between 30 and 60 years age and tobacco/pan masala chewers were taken. Molecular analysis of p53 codon 72 gene polymorphism was performed by polymerase chain reaction – restriction fragment length polymorphism for Arg/Arg, Arg/Pro, and Pro/Pro. Tissue expression of p53 was done by IHC. Results: Genotype frequencies of 35 carcinoma cases of p53 Arg/Arg, Arg/Pro, and Pro/Pro were 23%, 57%, and 20%, respectively, and six leukoplakia cases of p53 Arg/Arg and Arg/Pro genotype were 50% and 50%, respectively. By IHC for expression of p53 out of 35 cases of OSCC biopsies, 17 (48.57%) had weak staining, 14 cases (40%) showed evidence of p53 protein staining, and four cases (11.42%) showed negative staining. Among six cases of leukoplakia, 3 (50%) showed weak staining and 3 (50%) showed negative results. Conclusion: The findings of the study indicate that there is no significant association between p53 codon 72 gene polymorphism with OSCC and leukoplakia associated with tobacco/pan masala chewing.


How to cite this article:
Tandon N, Srivastava AN, Fatima N, Raza ST, Kumar V. p53 codon 72 gene polymorphism studies and p53 expression by immunohistochemistry in oral lesions as risk factor for malignancy.Int J App Basic Med Res 2017;7:243-246


How to cite this URL:
Tandon N, Srivastava AN, Fatima N, Raza ST, Kumar V. p53 codon 72 gene polymorphism studies and p53 expression by immunohistochemistry in oral lesions as risk factor for malignancy. Int J App Basic Med Res [serial online] 2017 [cited 2021 Sep 19 ];7:243-246
Available from: https://www.ijabmr.org/article.asp?issn=2229-516X;year=2017;volume=7;issue=4;spage=243;epage=246;aulast=Tandon;type=0