International Journal of Applied and Basic Medical Research

ORIGINAL ARTICLE
Year
: 2016  |  Volume : 6  |  Issue : 1  |  Page : 28--30

Oral exfoliative cytology as a screening tool for iron overload in ß-thalassemia patients


Ajit Singh Rathore, Neha Keshri, Devi Charan Shetty, Saurabh Juneja 
 Department of Oral Pathology, I. T. S. Dental College, Ghaziabad, Uttar Pradesh, India

Correspondence Address:
Saurabh Juneja
Department of Oral Pathology, I. T. S. Dental College, Murad Nagar, Ghaziabad - 201 206, Uttar Pradesh
India

Background: Increased iron overload is frequent problem in thalassemia patients, and this is monitored by serum ferritin levels or chemical assessment of the iron levels in liver tissue. However, repeated monitoring of serum ferritin levels to assess the iron overload is an invasive procedure associated with practical problems. Aims: To use Perl俟Q製 Prussian blue reaction to evaluate the iron overload in beta-thalassemia patients by staining the oral cytosmears. Materials and Methods: The study comprised 35 patients diagnosed with beta-thalassemia. Cytosmears were prepared from exfoliated oral epithelial cells, fixed in 70% ethanol and stained with Perl俟Q製 Prussian blue stain for detection of blue colored granules in the cytoplasm. Results: 29/35 (82.9%) cases showed a positive reaction for Perl俟Q製 Prussian blue reaction while 6/35 (17%) cases did not show the presence of blue colored granules in the oral cytosmears. The presence of iron detected by Perl俟Q製 Prussian blue reaction correlated with serum ferritin level (P < 0.05). Conclusion: Perl俟Q製 Prussian blue reaction can be used to evaluate the iron overload in beta-thalassemia patients by staining the oral cytosmears. It is a simple and noninvasive method for assessment of iron overload in such patients.


How to cite this article:
Rathore AS, Keshri N, Shetty DC, Juneja S. Oral exfoliative cytology as a screening tool for iron overload in ß-thalassemia patients.Int J App Basic Med Res 2016;6:28-30


How to cite this URL:
Rathore AS, Keshri N, Shetty DC, Juneja S. Oral exfoliative cytology as a screening tool for iron overload in ß-thalassemia patients. Int J App Basic Med Res [serial online] 2016 [cited 2022 Jan 24 ];6:28-30
Available from: https://www.ijabmr.org/article.asp?issn=2229-516X;year=2016;volume=6;issue=1;spage=28;epage=30;aulast=Rathore;type=0