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  Indian J Med Microbiol
 

Figure 2: Human immunodeficiency virus-interacting proteins from primary cells and cell lines were detected by immunoblotting purified proteins were obtained from primary cells (dendritic cell, macrophages, or cluster difference 4 T-cells) or from cell lines (HEC-1A cells and HeLa cells expressing or not dendritic cell-Specific Intracellular adhesion molecule-3-Grabbing Non-Integrin molecule). Western blotting was carried out by recombinant protein gp160, human immunodeficiency virus-1NDK, or R5-human immunodeficiency virus-1. Biotinylated goat polyclonal antibodies, antimouse immunoglobulin G, and the bounded gp160 were detected with the secondary biotinylated anti-gp160. After exposure to chemiluminescent reagents, membranes were exposed to X-ray film for 5 min

Figure 2: Human immunodeficiency virus-interacting proteins from primary cells and cell lines were detected by immunoblotting purified proteins were obtained from primary cells (dendritic cell, macrophages, or cluster difference 4 T-cells) or from cell lines (HEC-1A cells and HeLa cells expressing or not dendritic cell-Specific Intracellular adhesion molecule-3-Grabbing Non-Integrin molecule). Western blotting was carried out by recombinant protein gp160, human immunodeficiency virus-1<sub>NDK</sub>, or R5-human immunodeficiency virus-1. Biotinylated goat polyclonal antibodies, antimouse immunoglobulin G, and the bounded gp160 were detected with the secondary biotinylated anti-gp160. After exposure to chemiluminescent reagents, membranes were exposed to X-ray film for 5 min