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  Indian J Med Microbiol
 

Figure 1: Western blot analysis of human immunodeficiency virus-interacting cluster difference 4 complex 10 μ g of total membrane protein extracts of activated peripheral blood lymphocytes were purified. Purified proteins were separated on sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to nitrocellulose membranes. Western blotting was carried out using mouse primary monoclonal antihuman cluster difference 4 antibody, recombinant protein gp160, human immunodeficiency virus-1NDK, or R5-human immunodeficiency virus-1 (identified by JRCSF). The bounded mAb anticluster difference 4 was labeled with biotinylated goat polyclonal antibodies antimouse immunoglobulin G and the bounded gp160 with the secondary biotinylated anti-gp160. After exposure to chemiluminescent reagents, membranes were exposed to X-ray film for 5 min

Figure 1: Western blot analysis of human immunodeficiency virus-interacting cluster difference 4 complex 10 <b>μ</b> g of total membrane protein extracts of activated peripheral blood lymphocytes were purified. Purified proteins were separated on sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to nitrocellulose membranes. Western blotting was carried out using mouse primary monoclonal antihuman cluster difference 4 antibody, recombinant protein gp160, human immunodeficiency virus-1NDK, or R5-human immunodeficiency virus-1 (identified by JRCSF). The bounded mAb anticluster difference 4 was labeled with biotinylated goat polyclonal antibodies antimouse immunoglobulin G and the bounded gp160 with the secondary biotinylated anti-gp160. After exposure to chemiluminescent reagents, membranes were exposed to X-ray film for 5 min