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  Indian J Med Microbiol
 

Figure 1: Multiplex allele-specific-polymerase chain reaction for the codons 531, 526, and 516 of the rpoB gene. Wild-type 531 is indicated by band of 170 bp, wild-type 526 indicated by band of 185 bp, and wild-type 516 indicated by band at 218 bp. Isolates number H37Rv (positive control strain), 2447 and 3234 revealed wild types of the three codons 531, 526, and 516. Isolate number 2824 harbors mutations at 526 and 516. Isolates 2834, 3030, 4826, and 5546 harbors mutation in codon 531. Isolate number 3572 harbors a mutation in codon 526

Figure 1: Multiplex allele-specific-polymerase chain reaction for the codons 531, 526, and 516 of the rpoB gene. Wild-type 531 is indicated by band of 170 bp, wild-type 526 indicated by band of 185 bp, and wild-type 516 indicated by band at 218 bp. Isolates number H37Rv (positive control strain), 2447 and 3234 revealed wild types of the three codons 531, 526, and 516. Isolate number 2824 harbors mutations at 526 and 516. Isolates 2834, 3030, 4826, and 5546 harbors mutation in codon 531. Isolate number 3572 harbors a mutation in codon 526