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ORIGINAL ARTICLE
Year : 2020  |  Volume : 10  |  Issue : 2  |  Page : 86-90

Molecular detection of aspergillus in sputum of patients with lower respiratory tract infections


1 Department of Microbiology, Santosh Medical College, Ghaziabad, Uttar Pradesh, India
2 Department of Microbiology, UCMS, GTBH, New Delhi, India
3 Department of TB and Chest, Santosh Medical College, Ghaziabad, Uttar Pradesh, India

Correspondence Address:
Dakshina Bisht
Department of Microbiology, Santosh Medical College, Ghaziabad, Uttar Pradesh
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/ijabmr.IJABMR_364_18

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Background: Raised incidences of respiratory tract infections due to fungal agents in immunocompetent individuals are a cause of concern due to the unavailability of rapid diagnostic methods. Materials and Methods: Sputum and serum samples were collected from patients having lower respiratory tract infections (LRTIs), serum samples were screened for the presence of anti Aspergillus antibodies and sputum samples were homogenized and processed for identification of Aspergillus by conventional methods and further subjected to polymerase chain reaction (PCR) using genus-specific ITS 4-5 primers. Results: PCR identified Aspergillu s in 28% sputum samples, which was high as compared to conventional methods. Conclusion: Simple conventional PCR technique proves to be useful screening in for early identification of Aspergillus colonization in patients with LRTI, which can prevent irreversible damage in their lungs by fungal invasion.


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