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ORIGINAL ARTICLE
Year : 2018  |  Volume : 8  |  Issue : 2  |  Page : 100-105

Dissemination of multidrug-resistant, Class I and II integrons and molecular typing of CTX-M-producing Klebsiella pneumoniae


1 Department of Medical Microbiology, Nosocomial Infection Research Centre, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran
2 Nosocomial Infection Research Centre, Faculty of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran

Correspondence Address:
Ms. Azam Elahi
Department of Medical Microbiology School of Medicine, Kermanshah University of Medical Sciences, Shirudi Shahid Blvd, Daneshgah Street, 67148-69914, Kermanshah
Iran
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/ijabmr.IJABMR_333_16

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Introduction: Klebsiella pneumoniae (K. pneumoniae) is an important opportunistic pathogen causes serious community and hospital-acquired infections, which is highly resistant to antibiotics. We aimed to determine the frequency of multidrug resistant (MDR) and molecular typing of clinical isolates of K. pneumoniae. Methodology: One hundred isolates of K. pneumoniae were collected from clinical samples in three general hospitals in Kermanshah. The antimicrobial susceptibility and extended-spectrum beta-lactamases (ESBL) production of isolates were determined using disk diffusion and combined disk methods, respectively. The blaCTX-Mgene, class I and II integrons were detected using polymerase chain reaction. The blaCTX-Mpositive isolates were selected for genotyping using pulsed-field gel electrophoresis (PFGE). Results: MDR phenotype was observed in 56% of isolates. The 40% of isolates were ESBL positive and 35 isolates contained blaCTX-M. Class I and II of integrons were detected in 50 (89.2%) and 39 (69.6%) of MDR isolates, respectively. PFGE patterns of K. pneumoniae blaCTX-Mpositive isolates indicated 19 clusters (X1-19) with different genotype patterns. Conclusions: The study findings highlight the concern of circulating MDR strains of K. pneumoniae with blaCTX-Mand class I and II integrons in Kermanshah hospitals. The presence of integrons among isolates may facilitate the spread of new resistance genes in this bacterium. Therefore, surveillance for the spread of MDR strains of this bacterium is recommended in hospitals.


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