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ORIGINAL ARTICLE
Year : 2013  |  Volume : 3  |  Issue : 2  |  Page : 88-92

Evaluating the effectiveness of gel formulation of irradiated seed lectin Cratylia mollis during bone repair in rats


1 Nuclear Engeering Institute, Laboratory of Nanoradiopharmaceuticals, Rio de Janeiro, Brazil
2 Laboratory of Immunopathology Keizo Asami, Federal University of Pernambuco; Department of Morphology and Animal Physiology, Universidade Federal Rural de Pernambuco, Recife PE, Brazil
3 Department of Morphology and Animal Physiology, Universidade Federal Rural de Pernambuco, Recife PE, Brazil
4 Division of Experimental Surgery, Federal University of Pernambuco, Recife PE, Brazil
5 Department of Biochemistry, Federal University of Pernambuco, Recife PE, Brazil
6 Laboratory of Immunopathology Keizo Asami, Federal University of Pernambuco, Recife PE, Brazil

Correspondence Address:
Ralph Santos-Oliveira
Institute of Radiopharmacy Research, Zona Oeste Estadual University, Avenida Manuel Caldeira de Alvarenga, 1203 Campo Grande, Rio de Janeiro, State of Rio de Janeiro
Brazil
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/2229-516X.117062

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Context: Regeneration corresponds to the replacement of damaged cells with ones that have the same morphology and function. For experimental evaluation of materials that may favor the process of bone healing, defects are created with dimensions that prevent spontaneous regeneration. For the development and use of new drugs, it is necessary to study its effects in vitro, which depends on the formulation, concentration, and rate of irradiation in vivo and the route and frequency of administration; thus, it is possible to characterize the physiological and molecular mechanisms involved in the response and cellular effects. Objective: The objective of this study was to assess the effect of Cramoll-1,4 on the process of bone repair. Materials and Methods: A formulation of biopharmaceutical lectin Cramoll-1,4 at a concentration of 300 mg/100 mL was applied in a single application via gamma radiation and its effect on the process of bone repair in rats was assessed. Results: Histologically, it was observed that the bone defect is coated by loose connective tissue rich in fibroblasts, providing a range similar to the thick bone original and competing with site of new bone formation. This prevented direct contact between the formulation and experimental bone tissue, as, despite its proven effectiveness in experiments on the repair of skin lesions, the formulation used did not promote bone stimulation that would have promoted the tissue repair process. Conclusion: Because of the direct interference of loose tissue repair that prevented direct contact of the implant with the bone interface, the formulation did not promote bone stimulation.


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